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New imaging technology shows diabetes
Professor Ulf Ahlgren and his associates at the Umea Center for Molecular Medicine (UCMM) have subsequently elaborated the technology for biomedical imaging with optical projection tomography (OPT). Initially the method could only be used on relatively small preparations, but five years ago the scientists at Umea were able to adapt the technology to study whole organs including the pancreas from adult mice. The present findings describe a further development of the OPT technology by going from ordinary visible light to the near-infrared spectrum. Near infrared light is light with longer wavelengths that can more easily penetrate tissue. Thereby, the developed imaging platform enables studies of considerably larger samples than was previously possible. This includes the rat pancreas, which is important because rats as laboratory animals are thought to be physiologically more similar to humans.
|The enhanced technology allows new types of analyses, such as the possibility of evaluating preclinical samples for the purpose of developing better strategies for transplanting islets of Langerhans in diabetics. The image shows a liver from a mouse (gray) into which islets of Langerhans (blue) have been transplanted. By visualizing several markers in an organ it is possible to see directly where the islets of Langerhans wind up in the blood vessel tree.|
Internationally, huge resources are being committed to the development of non-invasive imaging methods for study of the number of remaining insulin cells in patients with developing diabetes. Such methods would be of great importance as only indirect methods for this exist today. However, a major problem in these research undertakings is to find suitable contrast agents that specifically bind to the insulin producing cells of the pancreas to allow imaging. In this context, the developed Near Infrared - OPT technology can play an important role as it enables the evaluation of new contrast agents. It may also be used as a tool to calibrate the non-invasive read out by e.g. magnetic resonance imaging (MRI). This is now going to be tested in the newly launched Marie Curie project “European Training Network for Excellence in Molecular Imaging in Diabetes,” which links together five major EU-funded research consortia with different cutting-edge competences in the field.
The study by scientists from Umea is presented in the Journal of Visualized Experiments, which is the first scientific journal to offer the video format for publication in the life sciences. Visualization in video presentations clearly facilitates the understanding and description of complex experimental technologies. It can help address two major challenges facing bioscience research: the low transparency and poor reproducibility of biological experiments and the large amounts of time and work needed to learn new experimental technologies.
Other authors of the article are Christoffer Svensson, Anna Eriksson, Abbas Cheddad, Andreas Hörnblad, Maria Eriksson, Nils Norlin, Elena Kostromina, and Tomas Alanentalo, all at UCMM; Fredrik Georgsson at the Department of Computer Science; all with Umea University, along with Antonello Pileggi, Miami University,
Florida, and James Sharpe at CRG, Barcelona, Spain.
For more information, please contact:
Professor Ulf Ahlgren, Umea Center for Molecular Medicine, Umea University
Phone: +46 (0)90-785 44 34
Link to this Webpage: